The hypothetical N-glycan charge: a number that characterizes protein glycosylation
نویسندگان
چکیده
منابع مشابه
The hypothetical N-glycan charge: a number that characterizes protein glycosylation.
The production of recombinant glycoprotein therapeutics requires characterization of glycosylation with respect to the lot-to-lot consistency. Here we introduce the ¿hypothetical N-glycan charge Z' as a parameter that allows to characterize the protein glycosylation in a simple, however, efficient manner. The hypothetical N-glycan charge of a given glycoprotein is deduced from the N-glycan mapp...
متن کاملProtein N-glycosylation and N-glycan trimming are required for postembryonic development of the pest beetle Tribolium castaneum
In holometabolous insects the transition from larva to adult requires a complete body reorganization and relies on N-glycosylated proteins. N-glycosylation is an important posttranslational modification that influences protein activity but its impact on the metamorphosis has not been studied yet. Here we used the red flour beetle, Tribolium castaneum, to perform a first comprehensive study on t...
متن کاملN-glycan analysis by CGE-LIF: profiling influenza A virus hemagglutinin N-glycosylation during vaccine production.
Glycoproteins, such as monoclonal antibodies as well as recombinant and viral proteins produced in mammalian cell culture play an important role in manufacturing of many biopharmaceuticals. To ensure consisting quality of the corresponding products, glycosylation profiles have to be tightly controlled, as glycosylation affects important properties of the corresponding proteins, including bioact...
متن کاملThe protein N-glycosylation in plants
larger antennae b(1, 2)-linked to the a(1, 6)or the a(1, 3)-mannose constitutive of the core have been called In plants, most proteins of the extracellular compartcomplex-type N-glycans, and the modified N-glycans ment and the endomembrane system are glycosylated having only an a(1, 3)-fucose and/or a b(1, 2)-xylose by N-linked oligosaccharides. The N-glycosylation of residues linked to the cor...
متن کاملUnraveling the mechanism of protein N-glycosylation.
Asparagine-linked glycosylation is the most ubiquitous protein co-translational modification in the endoplasmic reticulum (ER). The enzyme that catalyzes this process is called oligosaccharyl transferase (OT). It catalyzes the transfer of an oligosaccharyl moiety (Glc3Man9GlcNAc2) from the dolichol-linked pyrophosphate donor to the side chain of Asn within a consensus sequence of Asn-X-Thr/Ser,...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Glycobiology
سال: 1996
ISSN: 0959-6658,1460-2423
DOI: 10.1093/glycob/6.2.217